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Choudhuri,Jomuna V.; Mathor,Monica B.; Silva,Flávia H.; Han,Sang W.. |
Epidermal growth factor (EGF), which promotes epidermal regeneration and wound closure, is important for the proliferation and differentiation of epidermal and epithelial tissues in animals. Exogenous EGF is a promising therapeutic agent for wound healing, but its general use is restricted by the limited availability of this protein. In this work, we show that the transfection of mouse BALB/MK keratinocytes, which are totally dependent on EGF for growth and migration, with mature cDNA for human EGF via a retroviral vector abolished the cells requirement for exogenous EGF. The transformed cells had normal morphology and a growth rate that varied according to the source of the retroviral vector used. Keratinocyte transfection with EGF cDNA provides a time-... |
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Palavras-chave: BALB/MK keratinocytes; Epidermal growth factor; Retroviral vector. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500003 |
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Schwindt,Telma T.; Motta,Fabiana L.; Gabriela F.,Barnabé; Cristina G.,Massant; Guimarães,Alessander O.; Calcagnotto,Maria Elisa; Pesquero,João B.; Mello,Luiz E.. |
Cell therapy for neurological disorders has advanced, and neural precursor cells (NPC) may become the ideal candidates for neural transplantation in a wide range of diseases. However, additional work has to be done to determine either the ideal culture environment for NPC expansion in vitro, without altering their plasticity, or the FGF-2 and EGF mechanisms of cell signaling in neurospheres growth, survival and differentiation. In this work we evaluated mouse neurospheres cultured with and without FGF-2 and EGF containing medium and showed that those growth factors are responsible for NPC proliferation. It is also demonstrated that endogenous production of growth factors shifts from FGF-2 to IGF-1/PDGFb upon EGF and FGF-2 withdrawal. Mouse NPC cultured in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neural precursor cells; Neurosphere; Fibroblast growth factor 2; Epidermal growth factor; Differentiation. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300009 |
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